This category focuses on the core techniques of modern biotechnology that enable the analysis, modification, and amplification of genetic material. It covers PCR, cloning, and recombinant DNA technology, which form the foundation of genetic engineering, medical biotechnology, and molecular research. This section is suitable for Class 11–12 students, NEET aspirants, and undergraduate learners.
In this section, you will study:
Principles and steps of Polymerase Chain Reaction (PCR)
Enzymes, vectors, and host systems used in cloning
Steps involved in recombinant DNA technology
Restriction enzymes, ligases, and cloning vectors
Gene insertion, expression, and selection of recombinants
Applications of PCR and recombinant DNA in medicine, agriculture, and research
NCERT-based explanations, diagrams, flowcharts, MCQs, and exam-oriented questions
The content is structured to build clear conceptual understanding, strengthen process-based reasoning, and help students perform confidently in school examinations, NEET, and undergraduate assessments.
Develop a strong understanding of how molecular tools like PCR and recombinant DNA technology are applied to solve real-world biological problems.
Q. In recombinant DNA technology, what is the role of a vector?
A.
To cut DNA
B.
To amplify RNA
C.
To transport DNA into a host cell
D.
To visualize proteins
Solution
Vectors are DNA molecules used to deliver foreign genetic material into a host cell for replication.
Correct Answer:
C
— To transport DNA into a host cell
