Biotechnology Applications: PCR, Cloning, Recombinant DNA - Applications

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This section focuses on the practical applications of PCR, cloning, and recombinant DNA technology in medicine, agriculture, industry, and research. It is designed for Class 11–12 students, NEET aspirants, and undergraduate learners who want to connect molecular techniques with real-world uses.

In this section, you will study:

  • Use of PCR in disease diagnosis and forensic science

  • Applications of recombinant DNA technology in production of insulin, vaccines, and hormones

  • Role of cloning in gene expression studies and biotechnology research

  • Use of genetically modified organisms in agriculture

  • Application of molecular cloning in environmental and industrial biotechnology

  • Ethical, safety, and regulatory aspects of biotechnology applications

  • NCERT-based application questions, examples, and exam-oriented problems

The content is structured to enhance application-based understanding, strengthen practical reasoning, and help students confidently handle real-world and case-based questions in school exams, NEET, and undergraduate assessments.

Understand how PCR, cloning, and recombinant DNA technologies are applied to solve real-world problems in science, medicine, and agriculture.

Q. In cloning, what is the role of a vector?
  • A. To provide a template for DNA synthesis
  • B. To carry foreign DNA into a host cell
  • C. To amplify RNA
  • D. To degrade unwanted DNA
Q. In Mendelian genetics, what does the term 'homozygous' refer to?
  • A. Having two different alleles for a trait
  • B. Having two identical alleles for a trait
  • C. The physical expression of a trait
  • D. The genetic makeup of an organism
Q. What is recombinant DNA technology primarily used for?
  • A. To create genetically identical organisms
  • B. To produce proteins, such as insulin
  • C. To sequence entire genomes
  • D. To analyze gene expression
Q. What is the purpose of using restriction enzymes in recombinant DNA technology?
  • A. To amplify DNA
  • B. To cut DNA at specific sequences
  • C. To synthesize RNA
  • D. To clone entire genomes
Q. What is the significance of the 'denaturation' step in PCR?
  • A. To cool the reaction mixture
  • B. To separate the DNA strands
  • C. To synthesize new DNA strands
  • D. To add primers
Q. Which molecular technique is used to separate DNA fragments by size?
  • A. Gel electrophoresis
  • B. PCR
  • C. Cloning
  • D. DNA sequencing
Q. Which of the following is a common application of PCR?
  • A. Gene therapy
  • B. Forensic analysis
  • C. Protein purification
  • D. Cell culture
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