Sterilization and Disinfection - Numerical Applications

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This section focuses on numerical, quantitative, and data-based problems related to sterilization efficiency and microbial control. It is designed for Class 11–12 students, NEET aspirants, and undergraduate learners to strengthen their ability to apply sterilization concepts using calculations and logical reasoning.

In this section, you will practice:

  • Numerical problems based on microbial death rate and survival curves

  • Calculations involving temperature, time, and sterilization efficiency

  • Data interpretation questions on autoclave performance

  • Graph-based problems on thermal death curves

  • Numerical analysis of disinfectant concentration and exposure time

  • Table- and chart-based questions related to sterilization outcomes

  • NCERT-aligned numerical questions and exam-oriented practice sets

The content is structured to improve step-by-step analytical skills, enhance data interpretation, and help students confidently handle numerical and logic-based questions in school examinations, NEET, and undergraduate assessments.

Develop speed, accuracy, and confidence in solving numerical applications related to sterilization and disinfection through systematic practice.

Q. How long should items be exposed to ethylene oxide gas for effective sterilization?
  • A. 1 hour
  • B. 4 hours
  • C. 8 hours
  • D. 12 hours
Q. How many minutes of exposure to 160°C dry heat is required for sterilization?
  • A. 30 minutes
  • B. 60 minutes
  • C. 90 minutes
  • D. 120 minutes
Q. If a disinfectant has a contact time of 10 minutes, how many times can it be used in an hour?
  • A. 4 times
  • B. 5 times
  • C. 6 times
  • D. 7 times
Q. If a solution is diluted from 1000 ppm to 100 ppm, what is the dilution factor?
  • A. 1:10
  • B. 1:5
  • C. 1:20
  • D. 1:50
Q. If a sterilization cycle takes 30 minutes at 121°C, how long would it take at 134°C?
  • A. 10 minutes
  • B. 15 minutes
  • C. 20 minutes
  • D. 25 minutes
Q. What is the appropriate concentration of hydrogen peroxide for high-level disinfection?
  • A. 3%
  • B. 6%
  • C. 30%
  • D. 50%
Q. What is the D-value for a microorganism if it takes 5 minutes to reduce the population by 90% at 121°C?
  • A. 1 minute
  • B. 2 minutes
  • C. 5 minutes
  • D. 10 minutes
Q. What is the minimum effective concentration of ethanol for disinfection?
  • A. 50%
  • B. 70%
  • C. 90%
  • D. 100%
Q. What is the minimum effective temperature for autoclaving to ensure sterilization?
  • A. 100°C
  • B. 121°C
  • C. 130°C
  • D. 150°C
Q. What is the recommended contact time for a 10% bleach solution to effectively disinfect surfaces?
  • A. 1 minute
  • B. 5 minutes
  • C. 10 minutes
  • D. 15 minutes
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