Biotechnology Applications: PCR, Cloning, Recombinant DNA - Case Studies

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This section uses case studies and real-world scenarios to apply concepts of PCR, cloning, and recombinant DNA technology in analytical and practical contexts. It is designed for Class 11–12 students, NEET aspirants, and undergraduate learners to strengthen conceptual application and higher-order thinking skills.

In this section, you will study:

  • Case studies on PCR-based disease diagnosis and genetic testing

  • Scenarios involving cloning and expression of therapeutic proteins

  • Analysis of recombinant DNA techniques used in insulin and vaccine production

  • Case-based problems on selection and screening of recombinants

  • Situational studies on genetically modified organisms and biosafety

  • Interpretation of experimental workflows, data, and results

  • NCERT-based case studies and exam-oriented questions

The content is structured to improve analytical reasoning, enhance concept application, and prepare students for case-based and higher-order questions in school examinations, NEET, and undergraduate assessments.

Develop the ability to analyze real-world biotechnology applications by applying PCR, cloning, and recombinant DNA concepts with clarity and precision.

Q. In the context of gene expression, what is the role of mRNA?
  • A. To store genetic information
  • B. To transport amino acids
  • C. To carry the genetic code from DNA to ribosomes
  • D. To catalyze biochemical reactions
Q. What is a common method for visualizing DNA after gel electrophoresis?
  • A. Fluorescent microscopy
  • B. Ethidium bromide staining
  • C. Western blotting
  • D. Southern blotting
Q. What is recombinant DNA?
  • A. DNA that is naturally occurring
  • B. DNA that has been artificially created by combining DNA from different sources
  • C. DNA that is only found in viruses
  • D. DNA that is only found in bacteria
Q. What is the purpose of using restriction enzymes in molecular biology?
  • A. To amplify DNA
  • B. To cut DNA at specific sequences
  • C. To synthesize RNA
  • D. To visualize proteins
Q. Which type of mutation involves a change in a single nucleotide?
  • A. Frameshift mutation
  • B. Point mutation
  • C. Deletion mutation
  • D. Duplication mutation
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