Biotechnology Applications: PCR, Cloning, Recombinant DNA - Problem Set

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This problem set provides systematic and comprehensive practice on the concepts of PCR, cloning, and recombinant DNA technology. It is designed for Class 11–12 students, NEET aspirants, and undergraduate learners who want to strengthen their understanding through focused, exam-oriented problem solving.

In this section, you will practice:

  • Concept-based questions on PCR steps, enzymes, and reaction conditions

  • MCQs aligned with NCERT and competitive exam patterns

  • Assertion–reason and multi-statement problems

  • Diagram- and workflow-based identification questions

  • Numerical and data-interpretation problems

  • Application-based and mixed-difficulty questions

  • Progressive problem sets from basic to advanced levels

The problem set is structured to support gradual skill development, improve accuracy and confidence, and help students evaluate their preparation for school examinations, NEET, and undergraduate assessments.

Strengthen your mastery of PCR, cloning, and recombinant DNA technology through consistent and focused problem-solving practice.

Q. In cloning, what is the purpose of using a vector?
  • A. To provide a means of introducing foreign DNA into a host cell
  • B. To degrade unwanted DNA
  • C. To amplify RNA
  • D. To sequence DNA
Q. What is the primary function of PCR (Polymerase Chain Reaction)?
  • A. To amplify DNA sequences
  • B. To sequence DNA
  • C. To cut DNA at specific sites
  • D. To synthesize RNA
Q. What is the role of restriction enzymes in molecular biology?
  • A. To cut DNA at specific sequences
  • B. To synthesize RNA
  • C. To amplify DNA
  • D. To repair DNA
Q. What is the significance of gel electrophoresis in molecular biology?
  • A. To separate DNA fragments based on size
  • B. To amplify DNA
  • C. To synthesize proteins
  • D. To sequence RNA
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