Biotechnology Applications: PCR, Cloning, Recombinant DNA - Competitive Exam Level

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This section is designed for students preparing for competitive examinations where precision, conceptual depth, and speed are essential. It focuses on the most frequently tested and high-yield areas of PCR, cloning, and recombinant DNA technology, with strong emphasis on NCERT line-based understanding and exam-oriented practice.

In this section, you will study:

  • High-yield steps and enzymes involved in PCR and recombinant DNA technology

  • Tricky distinctions between cloning vectors, host systems, and selectable markers

  • NCERT-based MCQs on restriction enzymes, ligases, and DNA polymerases

  • Assertion–reason and multi-statement questions

  • Common conceptual traps and misconceptions in biotechnology processes

  • Previous year question patterns and exam-focused practice sets

The content is structured to improve accuracy, conceptual clarity, and exam temperament, helping students confidently tackle high-difficulty MCQs and theory-based questions in NEET and other competitive entrance exams.

Build a competitive edge by mastering PCR, cloning, and recombinant DNA technology through focused, exam-oriented preparation.

Q. What is the function of RNA polymerase during transcription?
  • A. To replicate DNA
  • B. To synthesize RNA from a DNA template
  • C. To splice RNA
  • D. To translate mRNA into protein
Q. Which of the following is a common application of cloning in biotechnology?
  • A. Gene therapy
  • B. DNA sequencing
  • C. Protein purification
  • D. All of the above
Q. Which technique is used to separate DNA fragments based on size?
  • A. PCR
  • B. Gel electrophoresis
  • C. Cloning
  • D. DNA sequencing
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