Biotechnology Principles and Processes

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Q. What is the function of restriction enzymes in biotechnology?
  • A. To cut DNA at specific sequences
  • B. To ligate DNA fragments
  • C. To replicate DNA
  • D. To transcribe RNA
Q. What is the main goal of gene therapy?
  • A. To treat genetic disorders
  • B. To enhance athletic performance
  • C. To create vaccines
  • D. To produce insulin
Q. What is the purpose of PCR (Polymerase Chain Reaction)?
  • A. To amplify DNA sequences
  • B. To sequence RNA
  • C. To purify proteins
  • D. To visualize chromosomes
Q. What is the role of plasmids in genetic engineering?
  • A. To serve as vectors for gene transfer
  • B. To provide energy to the cell
  • C. To synthesize proteins
  • D. To degrade foreign DNA
Q. Which enzyme is essential for the process of DNA replication?
  • A. DNA polymerase
  • B. RNA polymerase
  • C. Ligase
  • D. Restriction enzyme
Q. Which of the following is a common application of monoclonal antibodies?
  • A. Cancer treatment
  • B. Gene editing
  • C. DNA sequencing
  • D. Protein purification
Q. Which of the following is a common method for introducing DNA into a host cell?
  • A. Electroporation
  • B. Centrifugation
  • C. Filtration
  • D. Chromatography
Q. Which of the following is a key application of CRISPR technology?
  • A. Gene editing
  • B. Protein synthesis
  • C. Cell culture
  • D. Antibody production
Q. Which process is used to separate DNA fragments by size?
  • A. Gel electrophoresis
  • B. PCR
  • C. Cloning
  • D. Transcription
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