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In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?

Practice Questions

Q1
In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?
  1. Presence of proteins
  2. Presence of nucleic acids
  3. Presence of carbohydrates
  4. Presence of lipids

Questions & Step-by-Step Solutions

In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?
  • Step 1: Understand what UV-Vis spectroscopy is. It is a technique used to measure how much light is absorbed by a sample at different wavelengths.
  • Step 2: Know that different substances absorb light at specific wavelengths. This helps identify what is in the sample.
  • Step 3: Recognize that nucleic acids, like DNA and RNA, have a characteristic absorption peak.
  • Step 4: Learn that nucleic acids typically absorb light at a wavelength of 260 nm.
  • Step 5: Conclude that if you see a peak at 260 nm in your UV-Vis spectrum, it usually means that nucleic acids are present in the sample.
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